The HRD v2 Panel is designed for detection of SNVs, indels, and BRCA1 & BRCA2 exon level CNVs. 33 HRD-related genes can be assessed in either FFPE or blood samples. The panel utilizes proprietary Stem-Loop Inhibition-Mediated amplifi cation (SLIMamp®) technology, a tiled amplicon-based library prep chemistry for effi cient single-tube target enrichment.
| ARID1A | BRCA2 | ESR1 | FEN1 | PPP2R1A | RAD51D |
| ATM | BRIP1 | FANCA | KRAS | PTEN | RAD54L |
| ATR | CDK12 | FANCC | MRE11A | RAD50 | TP53 |
| BARD1 | CHEK2 | FANCD2 | NBN | RAD51 | |
| BRAF | CTNNB1 | FANCE | PALB2 | RAD51B | |
| BRCA1 | ERBB2 | FANCF | PIK3CA | RAD51C |
Genes marked in green indicate full CDS coverage
Maintain control of samples and results with single-tube, tiled amplification that can be performed in-house by any NGS lab.
Achieve variant detection as low as 1% VAF† without UIDs ‡ even with limited DNA input or poor sample quality.
Improve lab efficiency and reduce “no calls”, repeat testing, and difficult interpretation decisions
| Parameter | Description / Value |
|---|---|
| Enrichment chemistry | Multiplex PCR using tiled amplicons |
| Number of genes / amplicons | 33/1,314 |
| Number of targets | Full CDS coverage and exon-level CNVs for BRCA1 & BRCA2 genes, hotspots in 31 additional HRD-related genes; 114.5kb total size |
| Variant types | SNVs, indels, exon level CNVs for BRCA1 & BRCA2 |
| Average amplicon size | 157bp |
| Recommended DNA input range | 20ng to 80ng |
| Sample types | DNA from tissue, blood, or FFPE |
| Mapping rate | 97.9% ± 1.7% |
| % on-target aligned reads | 91.1% ± 0.8% |
| Coverage uniformity (% targets with >0.2X mean coverage) | 92.5% ± 0.9% |
| Recommended Reads Per Sample | ~6.5 million to 7.5 million paired-end reads |
*Mapping rate, % of on-target aligned reads, and coverage uniformity metrics are based on internal testing performed using reference standard materials.
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