Essential LBx Panel

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Essential LBx Panel

The Essential LBx panel is a robust NGS assay that interrogates 197 targets across 34 genes of interest from multiple solid tumor cancer types. This is a focused panel that can detect four types of variants from cell-free DNA (cfDNA) that has been extracted from plasma: single nucleotide variants (SNVs), small insertion/deletion (indel) variants, copy number amplification (CNA), and microsatellite instability (MSI). The panel uses proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp®) technology, a tiled amplicon-based library prep chemistry, designed by AI-empowered VersaTile™ Primer Design tool, for efficient single-tube target enrichment.

Essential LBx Panel (34 genes)

AKT1	CTNNB1	FGFR2	KIT	PPP2R1A	SF3B1
ALK	EGFR	FGFR3	KRAS	PTCH1	SMAD4
AR	▲ ERBB2	GNA11	▲ MET	PTEN	TERT (promoter)
ATM	ERBB3	GNAQ	NRAS	RAC1	TP53
BRAF	ESR1	GNAS	PDGFRA	RET
▲ CDK4	▲ FGFR1	HRAS	PIK3CA	RNF43

Copy Number Amplifications (CNAs) can also be detected in genes indicated by ▲. Genes marked in green indicate full CDS coverage

Panel Flow

Simple NGS library prep workflow

Maintain control of samples and results with single-tube, tiled amplification that can be performed in-house by any NGS lab.

Sensitive and robust chemistry

Achieve variant detection as low as 1% VAF† without UIDs ^‡ even with limited DNA input or poor sample quality.

Reduced fully-loaded lab costs

Improve lab efficiency and reduce “no calls”, repeat testing, and difficult interpretation decisions

Simple, one-day workflow

DNA sample

Library preparation

Sequencing

Data analysis

Reporting

Gene-specific PCR & cleanup

Indexing PCR & cleanup

Quantitation & normalization

Load sequencer

Panel Attribites

Parameter	Description / Value
Enrichment chemistry	Multiplex PCR using tiled amplicons
Number of genes / amplicons	34/201
Number of targets	Hotspots in 33 genes; full CDS coverage of TP53; 24 MSI sites; CNAs in 3 genes
Variant types	SNVs, indels, CNAs, and MSI
Average amplicon size	90bp
Recommended DNA input range	10ng to 30ng
Sample types	cfDNA from plasma
Mapping rate	≥90%
% on-target aligned reads	≥85%
Coverage uniformity (% targets with >0.2X mean coverage)	≥90%
~10 million paired-end reads	≥90%

*Mapping rate, % of on-target aligned reads, and coverage uniformity metrics are based on internal testing performed using reference standard materials.