Myeloid Panel

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Myeloid Panel

The Myeloid Panel is a robust NGS assay that interrogates 58 genes of interest* most relevant to myeloid cancers. The panel uses proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp®) technology, a tiled amplicon-based library prep chemistry, designed by AI-empowered VersaTile™ Primer Design tool, for efficient single-tube target enrichment.

Myeloid Panel (58 genes)

ABL1	BRAF	CEBPA	ETV6	HRAS	KDM6A	NPM1	PTEN	SMC1A	TP53
ANKRD26	CALR	CSF3R	EZH2	IDH1	KIT	NRAS	PTPN11	SMC3	U2AF1
ASXL1	CBL	CUX1	FLT3	IDH2	KMT2A	PDGFRA	RAD21	SRSF2	WT1
ATRX	CBLB	DDX41	GATA1	IKZF1	KRAS	PHF6	RUNX1	STAG1	ZRSR2
BCOR	CBLC	DNMT3A	GATA2	JAK2	MPL	PIGA	SETBP1	STAG2
BCORL1	CDKN2A	ETNK1	GNAS	JAK3	NF1	PPM1D	SF3B1	TET2

Genes marked in green indicate full CDS coverage

Panel Flow

Simple NGS library prep workflow

Maintain control of samples and results with single-tube, tiled amplification that can be performed in-house by any NGS lab.

Sensitive and robust chemistry

Achieve variant detection as low as 1% VAF† without UIDs ^‡ even with limited DNA input or poor sample quality.

Reduced fully-loaded lab costs

Improve lab efficiency and reduce “no calls”, repeat testing, and difficult interpretation decisions

Simple, one-day workflow

DNA sample

Library preparation

Sequencing

Data analysis

Reporting

Gene-specific PCR & cleanup

Indexing PCR & cleanup

Quantitation & normalization

Load sequencer

Panel Attribites

Parameter	Description / Value
Enrichment chemistry	Multiplex PCR using tiled amplicons
Number of genes / amplicons	58/766
Number of targets	Full CDS coverage of 18 genes, hotspots in 40 additional genes, FLT3 ITDs; 107.9kb total size
Variant types	SNVs, indels, ITD (internal tandem duplicates)
Average amplicon size	217bp
Recommended DNA input range	20ng to 60ng (20ng recommended)
Sample types	DNA from whole blood, PBMCs
Mapping rate	99.6% ± 0.2%
% on-target aligned reads	92.0% ± 5.3%
Coverage uniformity (% targets with >0.2X mean coverage)	96.8% ± 1.0%
Recommended Reads Per Sample	~4 million paired-end reads

*Mapping rate, % of on-target aligned reads, and coverage uniformity metrics are based on internal testing performed using reference standard materials.